hptlc-densitometry method development and validation for simultaneous determination of abacavir, lamivudine and zidovudine in combined dosage form

a simple, accurate and precise hptlc- densitometry method for the simultaneousdetermination of abacavir, lamivudine and zidovudine in pharmaceutical tablet has beendeveloped. separation of tablet components was performed on aluminum backed hptlc plates(silica gel 60 f254 20×20 cm with 0.2 mm thickness, camag, muttenz, switzerland ) usingmethanol : chloroform : acetonitrile (4 : 8 : 3 v/v). good sensitivity for all analytes was observedwith uv detection at 275 nm. the method was validated for linearity, accuracy, precision,specificity and robustness. the method allowed quantitation over the 200–1450 ng/band rangefor the three components. both linear and second order polynomial relationships were studied.second order polynomial fit was found to be more suitable and its residuals plot showed a muchbetter fitting than that of linear model, indicating good correlation and determinations (r =0.99995, 0.9998 and 0.9998 and r2 = 0.9999, 0.9997 and 0.9996 for 3-tc, abc and azt,respectively). the method has an accuracy of 99.35, 99.19 and 99.13 % for 3-tc, abc and aztrespectively. the method is robust and has the potential to determine these drug substancessimultaneously from the dosage forms.